Franchise of the Week

Bio-One Franchise Review 2026: Costs, Fees, Outlets, and Financial Performance

by Tariq Johnson

Table Of Contents

Section I – Bio-One Franchise Costs
Section II – Bio-One’s Initial Fees, Royalty Fees, and Marketing Fees
🛑 Is Your Territory Available?
Section III – Number of Franchised and Company-Owned Outlets
Franchised Outlets
Company-Owned Outlets
Total System Outlets
Section IV – News Updates & System Developments
Section V – Financial Performance Representations (Item 19, 2026 FDD)
Financial Performance Highlights
Summary and Next Steps
Real-World Reference Links

In this comprehensive review, you will explore the full legal disclosures, unit growth, fee structures, and financial performance for the Bio-One franchise system based on the company’s 2026 Franchise Disclosure Document (FDD) and official disclosures.

Section I – Estimated initial investment (franchise costs) for a Bio-One franchise, based on Item 7 of the company’s 2026 FDD
Section II – Initial franchise fee, royalty fee, brand fund, technology fee, and ongoing fees for a Bio-One franchise, based on Items 5 and 6 of the company’s 2026 FDD
Section III – Number of franchised and company-owned Bio-One outlets at the start and end of the year for 2023, 2024, and 2025, based on Item 20 of the company’s 2026 FDD
Section IV – News updates, leadership changes, system developments, and biohazard restoration industry growth trends
Section V – Presentation and analysis of Bio-One’s financial performance representations (average revenues and margins), based on Item 19 of the company’s 2026 FDD

Section I – Bio-One Franchise Costs

Estimated initial investment for a Bio-One franchise, based on Item 7 of the 2026 FDD:

Initial Franchise Fee: $60,000
Quick Start Package (Equipment, Tools, & Supplies): $39,500
Vehicle Lease/Purchase & Wraps: $5,000 to $18,000
Computer Hardware, Software, & POS Systems: $1,500 to $4,500
Initial Supplies, Safety Gear, & Chemicals: $3,000 to $8,000
Training Travel & Living Expenses: $1,500 to $3,500
Licenses, Permits, & Entity Formation: $500 to $2,500
Insurance Premiums (Initial Months): $3,000 to $8,000
Pre-Opening Marketing & Local Launch Promotion: $5,000 to $12,000
Additional Funds (3 Months): $15,645 to $44,095
Total Estimated Initial Investment: $134,645 to $221,095 (Varies based on territory size and initial operational setup).

Section II – Bio-One’s Initial Fees, Royalty Fees, and Marketing Fees

Fee disclosures based on Items 5 and 6 of the 2026 FDD:

Initial Franchise Fee: $60,000 for a single protected territory.
Quick Start Package Fee: $39,500 due at signing for initial specialty equipment, tools, and supplies.
Royalty Fee: 7.5% of Gross Sales, or a monthly minimum if greater ($750/month in months 1–12, $1,500/month in months 13–24, and $2,250/month from month 25 onward).
National Brand / Marketing Fund Contribution: 2% of Gross Sales (monthly minimum of $200; may increase up to 3%).
Local Marketing Requirement: The greater of $3,000 or 5% of Gross Sales per month.
Technology Maintenance Fee: $650 per month for proprietary job management, CRM tools, and system integrations.
Multi-Unit Discount: $10,000 discount off the initial franchise fee when acquiring more than one franchise simultaneously.
Veteran & First Responder Discount: 15% discount off the initial franchise fee for eligible military veterans and first responders.

🛑 Is Your Territory Available?

Biohazard, trauma, and specialty restoration territories across high-density markets sell out quickly. Before you spend weeks on brand due diligence, confirm whether your local market is open.

👉 [Click Here to Run a Territory Check in Your Area]

Section III – Number of Franchised and Company-Owned Outlets

Unit count and system status history based on Item 20 of the 2026 FDD:

Franchised Outlets

2023: Outlets at Start of Year: 118 | Outlets at End of Year: 122 | Net Change: +4
2024: Outlets at Start of Year: 122 | Outlets at End of Year: 125 | Net Change: +3
2025: Outlets at Start of Year: 125 | Outlets at End of Year: 127 | Net Change: +2

Company-Owned Outlets

2023: Outlets at Start of Year: 0 | Outlets at End of Year: 0 | Net Change: 0
2024: Outlets at Start of Year: 0 | Outlets at End of Year: 0 | Net Change: 0
2025: Outlets at Start of Year: 0 | Outlets at End of Year: 0 | Net Change: 0

Total System Outlets

2023: Outlets at Start of Year: 118 | Outlets at End of Year: 122 | Net Change: +4
2024: Outlets at Start of Year: 122 | Outlets at End of Year: 125 | Net Change: +3
2025: Outlets at Start of Year: 125 | Outlets at End of Year: 127 | Net Change: +2

Section IV – News Updates & System Developments

Backed by Five Star Franchising: Bio-One operates as a core brand within Five Star Franchising, a multi-brand franchisor platform backed by Princeton Equity Group. This structure provides franchisees with centralized marketing, call center routing, and corporate support.
Core Philosophy: Guided by its "Help First, Business Second" mission, Bio-One focuses on compassionate, professional service during high-stress events such as crime scene cleanup, hoarding remediation, suicide cleanup, and biohazard waste removal.
Recession-Resilient Demand: Operating within the broader $200B+ property restoration and specialty cleaning industry, Bio-One provides essential services funded largely through homeowner's insurance claims and institutional contracts, keeping demand consistent regardless of economic downturns.
B2B & Institutional Lead Streams: In addition to direct-to-consumer marketing, franchisees establish relationships with police departments, fire departments, victim advocates, property managers, and government agencies to secure direct referrals.

Section V – Financial Performance Representations (Item 19, 2026 FDD)

The Item 19 disclosure in the 2026 Bio-One FDD provides historical gross revenue and expense performance for reporting franchised operating units across the network.

Financial Performance Highlights

Average Unit Revenue: $372,962 across reporting franchised units.
Key Expense Structure:
- Payroll and Personnel: Represents the single largest operational expense line item, averaging approximately 35.2% of total revenue.
High Ticket & Margin Potential: Because services involve specialized biohazard containment, OSHA compliance, and insurance-backed claims, jobs carry high average invoice values and strong gross profit margins.
Low-Overhead Home-Based Footprint: Designed to operate without a retail lease or commercial storefront, keeping fixed monthly overhead low.

To learn more about the brand directly or verify territory availability, visit the official Bio-One website.

Summary and Next Steps

Bio-One offers a low-overhead, high-margin franchise opportunity in the specialized biohazard and trauma cleanup sector. Supported by Five Star Franchising, a initial investment starting under $135k, and strong demand driven by essential services, it provides an opportunity for mission-driven operators.

Ready to transition from employee to empire builder? [Apply for a Franchise Empire Consultation] today and let our team map out your personalized acquisition strategy.